Table 2.
Sequences of oligonucleotides primers used for PCR amplification
| Primer | Sequences (5'-3') | Positiona |
|---|---|---|
| L1 | AGTCCAGTCATCTCCATCCG | 1,562-1,581 |
| L2 | ACGATTTGGCTGTGCTGTAG | 1,730-1,749 |
| L3 | CTGTCTTAAGGTTAGGGCTGGC | 4,532-4,553 |
| L4 | GTAGGAAATATTGAGCCGAG | 4,826-4,845 |
| L5 | TTCTGATGTTTTGGCAAGCC | 7,578-7,597 |
| L6 | CAGAATGGCGCTTTGTTTGG | 7,669-7,688 |
| L7 | TTGAAGATAGGTTGCTCGTAG | 11,272-11,292 |
| L8 | ATACAGGAAAATTAACGAT | 11,367-11,385 |
| L9 | ATGTAGCAGTTTGTTCAAAC | 14,193-14,212 |
| L10 | AAGATGAGTCAACACCGAAGG | 14,445-14,465 |
| L11 | TATTCCATCCAGTTGCTCCC | 17,732-17,751 |
| L12 | CTTGTAAAGCTGGCCGCTAC | 17,978-17,997 |
| L13 | CGATCTGCTTTCGCTTTCCG | 21,725-21,744 |
| L14 | TAGCTGGTATGGCAACAATG | 21,895-21,914 |
| L15 | GAAGTTAACGGAGGAAGTATT | 26,043-26,063 |
| L16 | GCATATAATTACTACGCAACC | 26,165-26,185 |
| L17 | GTCATCCTTGTTATGTTGA | 29,697-29,715 |
| L18 | CCTACTTGGTGGTCGGCCA | 29,795-29,813 |
| L19 | CAGGATTTGATAACTAACC | 34,266-34,284 |
| L20 | ATAAGCGCACTAGATGGCAG | 34,473-34,492 |
| L21 | TGAACGGACCTTTGCTAATGAC | 37,793-37,813 |
| L22 | GAGGGGTGGTACTGGTTCCG | 38,120-38,139 |
| L23 | CCTACAATAACCTGGGAACT | 40,770-40,789 |
| L24 | GATCTTGTCCGATGGGGATG | 40,949-40,968 |
| L25 | ATGGGACAGTCCCTACCGTTGGCCTCGATTCAAAGCTTCTCAG | 1-43 |
| L26 | GGACTGCAGGCCTTTCAACGGCCCCTGATATGGCTACTATGTC | 18,716-18,758rb |
| L27 | GCCAGCCCTAACCTTAAGACAG | 4,532-4,553r |
| L28 | CAACCCCGCCCACAATAAGC | 4,043-4,062 |
| L29 | GCTTACATGCTTTTCCCCGC | 8,712-8,731r |
| L30 | CGAACCGTCACAGTCTGCAG | 8,592-8,611 |
| L31 | GCCGCGCAGTAAGTACTCAG | 13,315-13,334 |
| L32 | TGCCGATATCATTGGTTCAT | 13,212-13,231 |
| N1 | TATAGGTTT(C/A)TGTT | NPc |
| N2 | CTTTTGGAGCTG | NP |
| N3 | GAATGTGA(A/G)AA | NP |
| N4 | CATGTCTGCCGA | NP |
a the position is according to the sequence of the whole fragment of 42,897 bp.
b r indicates the reverse direction.
c NP indicates no prediction.