TABLE 4.

IFN-γ mRNA IN LUNG NEUTROPHILS AS DETECTED BY QUANTITATIVE PCR

Organism or Bacterial Component	Relative Amount of IFN-γ mRNA*
PBS	1.0 ± 0.0, n = 18
S. pneumoniae	15.9 ± 0.9,† n = 24
S. aureus	37.7 ± 2.8,‡ n = 6
E. coli	39.0 ± 5.7,‡ n = 21
P. aeruginosa	26.2 ± 7.1,‡ n = 12
LPS	7.2 ± 1.2,† n = 15
LTA	19.2 ± 0.7,† n = 6

Definition of abbreviations: E. coli = Escherichia coli; LTA = lipoteichoic acid; P. aeruginosa = Pseudomonas aeruginosa; PBS = phosphate-buffered saline; S. aureus = Staphylococcus aureus; S. pneumoniae = Streptococcus pneumoniae.

IFN-γ mRNA or 28S rRNA (internal control) in each sample of lung neutrophils was detected by quantitative PCR. Data are expressed as the fold increase in IFN-γ mRNA expression compared with PBS-treated control. Data represent means ± SEM of IFN-γ mRNA levels in lung neutrophils. Statistical significance was determined by analysis of variance with the Scheffé post hoc test. The number of mice (n) is provided. For each stimulus, three to eight separate experiments were performed.

^*PBS = 1.

^†p < 0.05 versus PBS group.

^‡p < 0.001 versus PBS group.