TABLE 5.
microRNAs THAT TARGET THE 3′ UNTRANSLATED REGION OF IFN-γ mRNA AND ARE EXPRESSED IN LUNG NEUTROPHILS*
| miRNA | Binding Site of miRNA on 3′ UTR of IFN-γ mRNA | miRNA Gene Location | P Value Comparing S. pneumoniae and E. coli |
|---|---|---|---|
| miR-15a | 376 | 14: 62250864–62250947 [–] | 0.046 |
| miR-15b | 375 | 3: 68813694–68813757 [+] | 0.040 |
| miR-26a | 36 | 9: 118940914–118941003 [+] | 0.021 |
| miR-26b | 37 | 1: 74440884–74440968 [+] | 0.019 |
| miR-29a | 503 | 6: 31012660–31012747 [–] | 0.008 |
| miR-30b | 393 | 15: 68168977–68169072 [–] | 0.008 |
| miR-106b | 410 | 5: 138606965–138607046 [–] | 0.031 |
| miR-424 | 376 | X: 50407432–50407526 [–] | 0.043 |
| miR-16 | 376 | 14: 62250717–62250809 [–]3: 68813824–68813918 [+] | P ≥ 0.05 |
| miR-17 | 408 | 14: 115442893–115442976 [+] | P ≥ 0.05 |
| miR-27a | 247, 531 | 8: 86732571–86732657 [+] | P ≥ 0.05 |
| miR-27b | 247, 531 | 13: 63402020–63402092 [+] | P ≥ 0.05 |
| miR-29b | 506 | 6: 31013023–31013093 [–] | P ≥ 0.05 |
| miR-29c | 503 | 1: 196863741–196863828 [+] | P ≥ 0.05 |
| miR-93 | 412 | 5: 138606751–138606838 [–] | P ≥ 0.05 |
| miR-99b | 271 | 17: 17967152–17967221 [+] | P ≥ 0.05 |
| miR-186 | 226 | 3: 157207243–157207313 [+] | P ≥ 0.05 |
Definition of abbreviations: E. coli = Escherichia coli; miRNA = microRNA; S. pneumoniae = Streptococcus pneumoniae; UTR = untranslated region.
P values were calculated by comparing the ΔCt values by analysis of variance and Scheffé post hoc test. Data represent means ± SEM of microRNA levels in lung neutrophils from nine mice studied in three separate experiments.