TABLE 6.
EXPRESSION OF microRNAs IN LUNG NEUTROPHILS DURING PNEUMONIA
| ΔCt |
Relative Amount of miRNA |
Percentage Difference in E. coli Compared with S. pneumoniae | |||||
|---|---|---|---|---|---|---|---|
| miRNA | PBS | S. pneumoniae | E. coli | PBS | S. pneumoniae | E. coli | |
| miR-15a | 4.59 ± 0.04 | 4.48 ± 0.11 | 4.19 ± 0.06*† | 1.00 ± 0.03 | 1.10 ± 0.09 | 1.32 ± 0.06* | 22 |
| miR-15b | 2.09 ± 0.03 | 2.14 ± 0.13 | 1.03 ± 0.49† | 1.00 ± 0.02 | 1.00 ± 0.08 | 3.28 ± 1.05*† | 228 |
| miR-16 | −1.06 ± 0.02 | −1.05 ± 0.16 | −1.42 ± 0.12 | 1.00 ± 0.02 | 1.05 ± 0.13 | 1.32 ± 0.11 | 27 |
| miR-17 | 3.26 ± 0.02 | 3.56 ± 0.40 | 3.11 ± 0.31 | 1.00 ± 0.01 | 1.14 ± 0.34 | 1.30 ± 0.21 | 16 |
| miR-26a | 1.65 ± 0.03 | 1.90 ± 0.20 | 1.12 ± 0.24† | 1.00 ± 0.02 | 0.92 ± 0.14 | 1.60 ± 0.22*† | 68 |
| miR-26b | 3.80 ± 0.04 | 3.74 ± 0.16 | 3.15 ± 0.17*† | 1.00 ± 0.02 | 1.10 ± 0.13 | 1.66 ± 0.21*† | 56 |
| miR-27a | 4.05 ± 0.03 | 4.15 ± 0.14 | 3.83 ± 0.27 | 1.00 ± 0.02 | 0.96 ± 0.08 | 1.34 ± 0.25 | 38 |
| miR-27b | 5.13 ± 0.03 | 5.51 ± 0.32 | 5.06 ± 0.26 | 1.00 ± 0.02 | 0.92 ± 0.20 | 1.17 ± 0.17 | 25 |
| miR-29a | 3.42 ± 0.03 | 3.08 ± 0.19 | 2.46 ± 0.10*† | 1.00 ± 0.02 | 1.36 ± 0.20 | 1.98 ± 0.14*† | 64 |
| miR-29b | 6.83 ± 0.05 | 5.92 ± 0.05* | 5.99 ± 0.23* | 1.01 ± 0.04 | 1.90 ± 0.07* | 1.99 ± 0.34* | 9 |
| miR-29c | 7.81 ± 0.04 | 7.25 ± 0.14* | 6.97 ± 0.08* | 1.00 ± 0.03 | 1.53 ± 0.17* | 1.82 ± 0.10* | 29 |
| miR-30b | 1.50 ± 0.02 | 1.60 ± 0.09 | 0.95 ± 0.22*† | 1.00 ± 0.01 | 0.94 ± 0.06 | 1.59 ± 0.22*† | 65 |
| miR-93 | 1.57 ± 0.02 | 2.08 ± 0.26 | 1.27 ± 0.35 | 1.00 ± 0.02 | 0.80 ± 0.15 | 1.50 ± 0.28† | 70 |
| miR-99b | 6.60 ± 0.04 | 7.29 ± 0.53 | 6.55 ± 0.29 | 1.00 ± 0.03 | 1.04 ± 0.35 | 1.22 ± 0.25 | 18 |
| miR-106b | 4.22 ± 0.02 | 4.18 ± 0.15 | 3.60 ± 0.20*† | 1.00 ± 0.02 | 1.08 ± 0.12 | 1.65 ± 0.20*† | 57 |
| miR-186 | 8.82 ± 0.07 | 9.41 ± 0.23 | 8.75 ± 0.23 | 1.01 ± 0.05 | 0.74 ± 0.12 | 1.15 ± 0.15 | 41 |
| miR-424 | 5.18 ± 0.02 | 5.19 ± 0.14 | 4.81 ± 0.11† | 1.00 ± 0.01 | 1.03 ± 0.11 | 1.32 ± 0.10* | 29 |
Definition of abbreviations: E. coli = Escherichia coli; miRNA = microRNA; PBS = phosphate-buffered saline; S. pneumoniae = Streptococcus pneumoniae.
Data represent means ± SEM of microRNA levels in lung neutrophils from nine mice in each group (PBS, S. pneumoniae, E. coli). The ΔCt value was calculated by normalizing for the expression of snoRNA 202 in each sample. Statistical significance was determined by analysis of variance and Scheffé post hoc test for ΔCt values and the relative amounts of miRNA.
*
P < 0.05 versus the PBS group.
†
P < 0.05 versus the Streptococcus pneumoniae group.