Sedimentation behavior of SF3b155.
[35S]Methionine-labeled SF3b155 translated
in vitro from the cDNA clone pBT B2-Xen was fractionated
after sucrose gradient centrifugation, separated by SDS/PAGE, and
processed for autoradiography. Fraction numbers are indicated on top of
the lanes (the top of the gradient is on the left). Bars indicate the
peak positions of the reference proteins BSA (4.3S), catalase (11.3S),
and thyroglobulin (16.5S). R, reference proteins 205, 116, 97.4, and 66
kDa (from top to bottom). Unfractionated reticulocyte lysate is shown
on the right (IVT).