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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Anal Biochem. 2011 Apr 23;415(2):105–115. doi: 10.1016/j.ab.2011.04.025

Figure 5. Effects of an SK COOH-terminal His6-tag and deletion of Arg253-Leu260 on the affinity of SK for [5F]FFR-[Lys]Pg.

Figure 5

A. Fluorescence titrations (ΔF/Fo) of 15 nM [5F]FFR-[Lys]Pg as a function of total SK concentration ([SK]o) for native SK in the absence (●) and presence (○) of 10 mM 6-AHA. B. Titrations as in A with SK-His6 in the absence (▲) and presence (△) of 10 mM 6-AHA. C. Titrations as in A with SKΔ (R253-L260) ΔK414-His6 in the absence (■) and presence (□) of 10 mM 6-AHA. Solid lines represent the non-linear least-squares fits to the data by the quadratic binding equation with the parameters in Table 1. Titrations were performed and analyzed as described in Materials and methods.