FIG. 3.

Effect of pregnancy on the Ca²⁺-induced MLC₂₀-P in β-escin-permeabilized uterine arteries in long-term, high altitude hypoxic sheep. β-escin-permeabilized uterine arteries from nonpregnant and pregnant ewes acclimatized to high altitude hypoxia were stimulated with 10 μmol/L Ca²⁺. MLC₂₀-P was detected by Western immunoblot (as described in METHODS). Representative immunoblot (top) shows unphosphorylated MLC₂₀ and MLC₂₀-P induced by 10 μmol/L Ca²⁺ at indicated time points in one of the uterine artery rings isolated from pregnant sheep. Data are means ± SEM of tissues from 5 animals.