Table 1.
Subdomain | Conserved structural/functional regions | Sequencea | Function |
---|---|---|---|
Fingers | G | 113 STSAGYPY 120 | Template bindingb |
F | 153 PLVTYVKDELRSKTKVEQGKSRLIEA 178 | NTP and template binding | |
I | 107 LEALDL 112 | Template binding | |
II | 184 SVAMRMAFGNLYAAFHK 200 | Template binding | |
Palm | A | 229 LFAFDYTGYDAS 240 | 2′-OH of NTP and metal B binding |
B | 293 TSIFNSMINNLIIRTLLLKT 312 | Base of NTP and template binding | |
C | 323 MIAYGDDVIAS 333 | Primer and metal A binding and catalysis | |
D | 338 VDASLLAQSGKDYGLTMTPADKSAT 362 | Triphosphate of NTP binding and catalysis | |
E | 363 FETVTWENVTFLKRFFRA 380 | Nascent RNA 3′-end binding | |
Thumb | III | 405 KDPRNTQDHVRSLCLL 420 | Nascent RNA duplex binding |
The residues shown in boldface are completely conserved among RdRps; those residues representing signatures of the conserved sequence motifs are shown in boldface and underlined.18