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. Author manuscript; available in PMC: 2012 Jun 10.
Published in final edited form as: Biochem Biophys Res Commun. 2011 May 12;409(3):526–531. doi: 10.1016/j.bbrc.2011.05.038

Fig. 3. Proteolytic stability of recombinant PitB and PitBK63A K243A.

Fig. 3

Recombinant PitB (lanes 1–4) and PitBK63A K243A (lane 5–8) were treated either with trypsin (A) or added to a culture of S. pneumoniae strain GA47901 (B) prior to separation by SDS PAGE. Whether trypsin or culture was present in the reaction and whether the samples were boiled prior to incubation is indicated on top of the gels.