. Author manuscript; available in PMC: 2012 Jun 26.

Published in final edited form as: Vaccine. 2011 May 10;29(29-30):4709–4719. doi: 10.1016/j.vaccine.2011.04.090

Table 1.

Primers used for qRT-PCR quantification for cytokine transcription.

Gene	Forward primer	Reverse primer	Source^a
β-actin	GGAATCCTGCGGCATTCAC	GGATGTCGACGTCACACTTCA	NM173979
GAPDH	GCATCGTGGAGGGACTTATGA	GGGCCATCCACAGTCTTCTG	[59]
IL-10	GAGCAAGGCGGTGGAGAAGG	GATGAAGATGTCAAACTCACTCATGG	NM174088
IL-12p35	CAGCAAGCCCAGGAAGGA	TGACAGCCCTCAGCAGGTTT	NM174355
IL-17	GGACTCTCCACCGCAATGAG	TGGCCTCCCAGATCACAGA	NM001008412
IL-22	GAGGTGCTGTTCCCCCAAT	GAAGGGCACCACCTTTTCC	NM001098379
IL-23p19	CACAAGGACTCAGGGACAACAG	AAAAACCAGGCCTCGATGAA	XM588269
Gran^b	GACCTGCTGCTCCAAGGAGAA	CCAACTTGTCCATCAGATGTTGTATT	NM001075143
Foxp3	CCGGCATGCCTAGGAAAGA	ACGCCATTTGCTAGCAGTGAGT	DQ322170
IFN-γ	CTTGAATGGCAGCTCTGAGAAA	TGCAGATCATCCACCGGAAT	[60]
T-bet	GAGGCGGCCTGCAACAC	CGGCGATGAACTGGGTTT	XM583748
TGF-β	CATCTGGAGCCTGGATACACAGT	GAAGCGCCCGGGTTGT	[60]
TNF-α	TCTACCAGGGAGGAGTCTTCCA	GTCCGGCAGGTTGATCTCA	[61]
RORC	GAGTGCCTTGCGCTTTTCA	GATGAGGACGAGGGCTGTG	DQ667051

Gene accession no. is given for the primers developed in this study.

Granulysin.