Fig. 8.

EPO and CEPO decrease microglial activation after white matter injury. We performed immunohistochemistry for the microglial marker CD68 48 h after H/I or H/I/L on mice treated with. vehicle, EPO (40 µg/kg, i.p.) or CEPO (40 µg/kg, i.p.). (A–D) H/I mice exhibited CD68⁺ activated microglia in the white matter. Treatment with EPO (B) or CEPO (C) did not diminish the presence of these cells compared with vehicle-treated mice (D). (E–H) In contrast, H/I/L mice showed significant microglial recruitment and CD68-immunoreactivity in the white matter (E), and treatment with EPO (F) or CEPO (G) significantly reduced the number of CD68⁺ cells. Quantification in the white matter track confirmed this (H, *, p<0.05). Scale bar, 10 µm.