Table 1. Differences in corneal layer boundary segmentation between two manual graders for 40 B-scans (Column I), as compared to the position differences between the automatic segmentation and the expert manual grader of the same 40 B-scans (Column II). Each pixel is approximately 3.4 µm in the cornea. The automatic segmentation has the same or lower mean difference and standard deviation compared to the expert than another trained manual grader.

	Comparison of Two Manual Graders		Comparison of Automatic and Manual Expert Segmentation
	Column I		Column II
Corneal Layer Boundary	Mean Difference (pixels)	Standard Deviation (pixels)	Mean Difference (pixels)	Standard Deviation (pixels)
Epithelium-Air Interface	1.5	0.4	0.6	0.4
Epithelium-Bowman Interface	1.2	0.5	0.9	0.5
Endothelium-Aqueous Interface	2.1	2.3	1.7	2.1