Figure 1.

GLPG0187 affects osteoclastogenesis. (A) Schematic representation of osteoclast formation in an osteoclast model adapted from Jurdic et al. [44]. (B) Representative images of light microscopy and fluorescent actin staining showing the different morphology of differentiating osteoclasts. (C) Effect of GLPG0187 on the amount of osteoclasts in each differentiation morphology. (D) Bone marrow cells isolated from 8-week-old female mice tibiae and femora were cultured during 5 days in the presence of RANKL and mM-CSF and of various concentrations of GLPG0187. TRAcP activity was measured using densitometry. Data are expressed as mean ± SEM (n = 4). *P < .05 versus stimulated control.