Figure 3.
Effect of GLPG0187 on EMT, migration, and percentage of ALDHhi stem/progenitor cells. Cells were treated for 48 hours with a concentration range of GLPG0187, and subsequently, relative E-cadherin/vimentin ratio (A) was measured with flow cytometry and levels of EMT inducers including Snail1 and 2 and Twist were measured with real time qPCR (B). Data are represented as log fold change (log2-ΔΔCt). Mean numbers of migrated PC-3M-Pro4/luc cells per field were measured (C). Data are presented as mean ± SEM (*P < .05) and are representative for three independent experiments. Percentage of PC-3M-Pro4/luc cells with high ALDH activity as measured with ALDEFLUOR assay (D) [4].