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. 2011 Jun;13(6):526–536. doi: 10.1593/neo.11182

Figure 1.

Figure 1

Androgen depletion induces senescence in prostate cancer cells. (A) Cytochemical detection of SA-β-gal activity in LNCaP cells cultivated in presence (FBS) or absence (CS) of androgens for 2 or 16 days (2D, 16D). (B) Quantitative analysis of SA-β-gal activity in LNCaP and LAPC-4 cells using fluorescent substrate. *Statistically significant changes (P < .05) compared with FBS-treated cells. (C) TRAP assay to detect telomerase activity in LNCaP cells. (D) Immunofluorescent detection of HP1β and P-H2AX (S139) in LNCaP cells. White arrows and arrowheads indicate P-H2AX (S139) staining and HP1β-positive foci, respectively.