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. 2011 Jun;13(6):526–536. doi: 10.1593/neo.11182

Figure 2.

Figure 2

Analysis of markers of the senescent secretome and of NED after androgen depletion. (A) Flow cytometric analysis of IGFBP3 in LNCaP and LAPC-4 cells cultivated for 8 days under the growth conditions indicated (see Materials and Methods). Histogram represents typical result obtained by flow cytometric analysis. Error bars, MFI ± SD from two independent experiments. (B) Cathepsin B concentration, determined by ELISA, in medium conditioned by LNCaP or LAPC-4 cells cultivated for 8 days with or without androgen. Error bars, mean ± SD from four experiments. *Statistical significance (P < .05). (C) Flow cytometric analysis of serotonin in LNCaP and LAPC-4 cells and (D) histamine in LAPC-4 cells cultivated for 8 days with or without androgen. Error bars, MFI ± SD from two independent experiments.