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MLN4924-induced senescence is irreversible. Cells were treated with MLN4924 at 0.1 µM for 72 hours to induce senescence. Cells were washed and either cultured in drug-free medium for additional 72 hours (MLN-72h-washed) or were continuously cultured in drug-containing medium for additional 72 hours (MLN-144h), followed by photography using Olympus DP70 camera (x400) with size bar shown (A), colony staining (B), and immunoblot analysis (C).
