Figure 4.
Depolarization preconditioning protects against oxygen-glucose deprivation (OGD). A. Brightfield micrographs of trypan blue staining from each condition: (left to right) control dish 24 hr post-preconditioning with 30 mM NaCl and sham OGD; dish preconditioned with 30 mM NaCl, subjected to OGD for 2.5 hr; dish preconditioned with 30 mM KCl, then subjected to OGD for 2.5 hr. B. Summary graph showing protection afforded by KCl depolarization preconditioning (n=4). Asterisks denote p < 0.05 compared with the NaCl OGD condition (Bonferroni corrected t-tests). Gray bar emphasizes the major hypothesized result of KCl preconditioning protection.