Figure 1.

Overexpression of Xfz3 promotes ectopic eye formation and causes proximal eye defects. (a) Stage 42 embryo showing an ectopic eye in the dorsal head region (white arrow). (Inset) A higher magnification view of the ectopic eye. (b) Stage 45 embryo with an ectopic eye in the dorsal midline of the head. (c) Stage 42 embryo showing streaming of the retinal pigment epithelium from the endogenous eye toward the midline. (d) Stage 45 embryo with abnormally positioned eye on the injected side, marked with β-galactosidase (blue). (e) Section through an embryo similar to the one shown in d, showing the eye adjacent to the neural tube on the injected side (Left). (f) Section through a stage 45 embryo, showing an ectopic eye in the roof of the fourth ventricle that is fully labeled with the tracer β-galactosidase (blue). (Inset) A high-magnification view from an adjacent section, showing a row of morphologically distinct photoreceptor outer segments in the ectopic eye (arrowheads). (g and h) Immunolabeling of a section through a normal eye (g) and ectopic eye (h) with anti-Pax6 antibodies (red) and anti-rhodopsin antibodies (green). Nuclei are labeled with Hoechst (blue). The ectopic eye in h shows an arrangement of retinal layers similar to that in the normal eye in g.