Figure 2.

Overexpression of Xfz3 promotes ectopic expression of genes regulating eye development. A whole-mount in situ hybridization analysis was made of stage 14 embryos (a–f) that were either uninjected (a, c, and e) or Xfz3 injected (b, d, and f) and then labeled for Xpax6 (a and b), Xrx (c and d), or Xotx2 expression (e and f). Arrows indicate modest expansion of Xrx or Xotx2 expression (d and f). Stage 28 embryos (g–l) are either uninjected (g, i, and k) or Xfz3 injected (h, j, and l) and then labeled for Xpax6 (g and h), Xrx (i and j), or Xotx2 expression (k and l). Arrows indicate ectopic expression in injected embryos (h, j, and l). β-Galactosidase (sky blue) marks the regions of the embryo derived from the injected blastomere.