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. 2004 Jan 15;113(2):200–208. doi: 10.1172/JCI19639

Figure 2.

Figure 2

Deposition of IgG in the brain. (a) Immunofluorescent staining with anti-GA2 antibody (green) and nuclear staining with PI (red) is shown for frozen brain sections. Both cell surface expression and intracellular storage of GA2 in the cerebella of 14-week-old Hexb+/+ mice and Hexb–/– mice are shown (left panel). ML, molecular layer; Pc, Purkinje cells; GL, granular layer. The presence of IgG detected by anti-IgG antibody (green) is shown in the same area (right panel). Representative sections with three mice per group are shown. IgG is detected only in Hexb–/– mice. Original magnification, ×400. (b) Immunohistochemical staining with alkaline phosphatase–labeled anti-IgG antibody shows IgG deposition in the neurons of the thalamus (red) of 14-week-old Hexb–/– mice with no IgG deposition evident in the thalamus of age-matched Hexb+/+ mice. Representative sections are shown. Methylgreen was used for counterstaining. Original magnification, ×200. (c) Deposition of IgG present in the hippocampal commissure of BBB-disrupted SCID mice following injection of 14-week-old Hexb–/– mice serum. Prior to injection, the presence of the GA2 (stained green) was confirmed in SCID mice (left). Deposition of IgG was investigated following BBB disruption. Serum transfer from the Hexb+/+ mice did not reveal IgG deposition (middle), whereas the presence of IgG was demonstrated in the same area of the SCID mice injected with Hexb–/– mice sera (right). Green, presence of Alexa-labeled GA2 (left) or Alexa-labeled IgG (middle and right); red, nuclear staining with PI. Arrows indicate axons or dendrites that were stained green. Original magnification, ×400.