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. 2004 Jan 15;113(2):200–208. doi: 10.1172/JCI19639

Figure 4.

Figure 4

Analysis of ganglioside storage. (a) Immunohistochemical detection of GM2 in the cerebral cortex of a 14-week-old mouse. Both Hexb–/–FcRγ+/+and Hexb–/–FcRγ–/– mice, but not WT (Hexb+/+FcRγ+/+), showed GM2 storage in the cerebellum (stained green or yellow). PI was used for nuclear staining. (b) Extraction and analysis of brain gangliosides. Gangliosides were extracted from the brains of 14-week-old mice and loaded onto HPTLC plates. Both Hexb–/–FcRγ–/– and Hexb–/–FcRγ+/+ mice showed similar levels of GA2 and GM2 storage. M, bovine ganglioside marker.