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. 2011 May 21;60(6):1668–1676. doi: 10.2337/db10-1628

FIG. 3.

FIG. 3.

IFN-γ prevents SAG-induced inhibition of adipocyte differentiation via Jak-Stat signaling. A: Shh-LIGHT2 cells were treated with 200 nmol/L SAG and/or 25 ng/µL IFN-γ in the absence or presence of JakInh. Simultaneous treatment of cells with SAG and JakInh resulted in an additional increase in relative Gli1 luciferase activity compared with SAG only–treated cells. B–D: We also tested whether effects of IFN-γ on Gli1 expression could persist in MEFs from Ifngr1−/− and Stat1−/− mice. The inhibitory effect of IFN-γ on Shh signaling was cancelled in Ifngr1−/− and Stat1−/− MEFs. These experiments demonstrate that IFN-γ acts by means of the Jak-Stat pathway to abrogate the effects of Shh signaling. Data represent means ± SEM of triplicate samples. *P < 0.01; **P < 0.001; NS, not significant.