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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Dev Biol. 2011 Mar 4;353(1):38–49. doi: 10.1016/j.ydbio.2011.02.027

Fig. 4. Grhl2, E-cadherin, β-catenin and Ezrin expression in the lung epithelium.

Fig. 4

(A, B) Grhl2 RNA is detected in the lung epithelium at both E14.5 (A) and E18.5 (B).

(C–H) E18.5 wildtype (C, E, G) and Grhl21Nisw (D, F, H) lungs immunostained with antibodies against E-cadherin (C, D; E-CDH), β–catenin (E, F) or Ezrin (G, H) in red; green is phalloidin (PH). E-cadherin, β–catenin and Ezrin are all downregulated and/or mislocalized in the Grhl2 mutant lung. For A and B: Leica DM 5000B microscope and 10X air HC PlanApo objective, NA 0.40. For C–H Imaging was performed with LSM 510META lens c-Apochromat 40X/1.2 W. C–F 1.3X zoom; G and H 2.2X zoom.