Figure 1.
DNA Damage Induces the Expression of the KSRP-associated MiRNAs. (A) KSRP-dependent miRNAs are induced after DNA damage in an ATM-dependent manner. Atm+/+ and Atm−/− mouse embryonic fibroblasts were treated with NCS (200 ng/ml), and cells were harvested at indicated time points for microarray analyses. Green or red color on the heat map indicates a decrease or increase of miRNA level and color intensities correspond to relative signal levels on a logarithmic scale. (B) DNA damage has no significant effects on the primary transcript levels of the KSRP-dependent miRNAs. Expression levels of pri-miRNAs in the RNA samples were analyzed by quantitative RT-PCR and error bars represent the mean ± SD.