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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Mol Cell. 2011 Feb 18;41(4):371–383. doi: 10.1016/j.molcel.2011.01.020

Figure 3.

Figure 3

Activity of KSRP in Regulating miRNA Biogenesis is Dependent on the Predicted ATM Phosphorylation. (A) Protein sequence analysis reveals three conserved serines that are potentially phosphorylated by ATM in mammals. (B) A series of point mutations were generated on the predicted phosphorylation sites. (C) Wildtype, but not phosphorylation-deficient mutant KSRP restores DNA damage-induced miRNA biogenesis. KSRP-depleted U2OS cells were transfected with control vector or vector expressing shRNA-resistant wildtype KSRP or 3A mutant of KSRP. Cells were treated with NCS (500 ng/ml) 24 h after transfection and expression levels of mature miRNAs were determined by quantitative RT-PCR. Error bars represent the mean ± SD.