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Reverse phase high-peformance liquid chromatogram (RP-HPLC) chromatogram of (A) H. hypnale, (B) H. nepa and (C) H. zara using a Phenomenex Jupiter C18 column (5μ 300A 250x4.6mm) with mobile phase 15% (v/v) acetonitrile containing 0.1% (v/v) trifluoracetic acid, increasing to 70% acetonitrile by t=100min, at a flow rate of 0.5ml/min.
