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. 2011 May 17;50(24):5443–5452. doi: 10.1021/bi200369p

Table 2. Kinetic Parameters of Heme Binding and Transfer.

		off rate ± SE (×10^–3 s^–1)
	K_D ± SE (heme; μM)	k_fast	k_slow	k_transfer ± SE (s^–1)	K_D^a ± SE (μM)
IsdB-N2 WT	<0.38 ± 0.06	28 ± 3	4.8 ± 0.02	81.5 ± 5.7	18.6 ± 3.2
IsdB-N2 M362L	<0.49 ± 0.09	130 ± 11	13 ± 0.04	34.4 ± 2.4	16.5 ± 3.5

^a

Estimated dissociation constant for the protein complexed with IsdA-N1.