Table 1.

Primers used for PCR amplifications, identified by laboratory number

Primer	Position	Sequence
1407	P1 B/B	CTCAGGAATTCCACCATGAATGAAACAGAGTGC
4017	P2 B/B	CGCGGATCCATCGATGGGTGCG
4968	P1 B/S	ATCAGGATCCGCGGAGATTCGGATTGCG
4969	P2 B/S	ATCAGTCGACGCAGTCCTGGCAGAACGGC
4970	P1S/X	ATCAGTCGACCCGCTGCTCTTTTCGGTGC
4971	P2 S/X	ATCATCTAGAGTTAACTATGCATAATCTGGGACG
4018	MP2 48	GTCTTCTGTGCGAATAAGTTG
4019	MP1 48	CAACTTATTGGCACAGGAGAC
4020	MP2 133	CCAAGCATAATGGCCACGAAG
4021	MP1 133	CTTCGTGGCCATTATGCTTGG
4972	MP2 133/134	AGCATAATGGCCGCGAAGAAGGCCAAGTCCTTAATCG
4973	MP1 133/134	GCCTTCTTCGCGGCCATTATGCTTGGGTAGGTGAC
4974	MP2 325	GTGAGAGCGACGAGAGCCATCAACCAGGG
4975	MP1 325	GCTCTCGTCGCTCTCACGGCAGCCCGGTAG
4976	MP2 335	GCTTCAAGGCCACCAAGGGTTCCAAGGGCATCG
4977	MP1 335	CCCTTGGTGGCCTTGAAGCCCTGGTTGATGGC
4978	MP2 352	AACGCCTCGCATTGCGCATACATAGAGGACGAG
4979	MP1 352	ATGCGCAATGCGAGGCGTTGCTCCTCGAAGC
4980	MP2 393	AGTACGCCGCTAGGCGACTGCACCACCACG
4981	MP1393	AGTCGCCTAGCGGCGTACTTCGACAACCTCTC
4982	MP2 598	AGTCCGTCGCCTTGAAGAGCTCCCGCCGGGG
4983	MP1598	CTCTTCAAGGCGACGGACTGGCGGGAGCAG
4984	MP2 601/602	CCTTGAAGGCGGCCCGCCGGGGATCTGACATGTC
4985	MP1 601/602	CCCCGGCGGGCCGCCTTCAAGGAGACGGACTGGC

P denotes flanking primer, where P1 represents the primer binding to the very 5′ end, and P2 represents binding to the very 3′ end of the restriction fragment; MP denotes mutagenesis primer, with MP1 primers allowing amplification in 5′–3′ direction, and MP2 primers allowing amplification in 3′–5′ direction. Amplifications were performed on restriction fragments of DmOctα1Rb: BamHI (B)/B, B/SalI (S), and S/XbaI (X). Positions of mutated amino acids are indicated with the respective MPs. Nucleotide sequence of primers is in 5′–3′ direction. Triplets encoding the modified residues are underscored.