Table 3.
Mascot data for the subgroup of proteins identified as belonging to the GO biological function category “glycolysis”
| Gene | SwissProt | Description | Mascot score | Mass (Da) | Peptides | Coverage (%) |
|---|---|---|---|---|---|---|
| ALDOA | ALDOA_RAT | Fructose-bisphosphate aldolase A | 494 | 39,327 | 16 | 48.1 |
| ENO1 | ENOA_RAT | α-Enolase | 451 | 47,098 | 16 | 37.4 |
| ENO3 | ENOB_RAT | β-Enolase | 374 | 46,984 | 14 | 32.5 |
| GAPDH | G3P_RAT | Glyceraldehyde-3-phosphate dehydrogenase | 1150 | 35,805 | 47 | 69.1 |
| GPI | G6PI_RAT | Glucose-6-phosphate isomerase | 151 | 62,787 | 4 | 5.6 |
| Hk1 | HXK1_RAT | Hexokinase-1 | 71 | 102,342 | 2 | 2.9 |
| PKM2 | KPYM_RAT | Pyruvate kinase isozymes M1/M2 | 840 | 57,781 | 29 | 52.9 |
| LDHA | LDHA_RAT | l-Lactate dehydrogenase A | 282 | 36,427 | 12 | 36.5 |
| LDHB | LDHB_RAT | l-Lactate dehydrogenase B | 633 | 36,589 | 24 | 49.6 |
| MDH2 | MDHM_RAT | Malate dehydrogenase, mitochondrial | 807 | 35,661 | 22 | 66.3 |
| OGDH | ODO1_RAT | 2-Oxoglutarate dehydrogenase, mitochondrial | 396 | 116,221 | 19 | 15.3 |
| DLAT | ODP2_RAT | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, | 490 | 67,123 | 18 | 31.1 |
| PDHA1 | ODPA_MOUSE | Pyruvate dehydrogenase E1 component subunit α, somatic form, mitochondrial | 375 | 43,204 | 20 | 37.2 |
| PDHB | ODPB_RAT | Pyruvate dehydrogenase E1 component subunit β, mitochondrial | 444 | 38,957 | 15 | 35.3 |
| PGK1 | PGK1_RAT | Phosphoglycerate kinase 1 | 95 | 44,510 | 7 | 19.8 |
| TPI1 | TPIS_RAT | Triosephosphate isomerase | 95 | 26,832 | 6 | 22.5 |
Each protein was identified multiple times in the 3 mass spectrometry experiments performed. Representative examples for various proteins for a given experiment are listed. Peptides column shows the number of peptide matches with a protein; Coverage column shows the percentage of the protein amino acid sequence matched by the peptides.