Figure 2.

FITC-dextran diffusion in the ASL in unpertubed, well-differentiated primary cultures of human bronchial epithelial cells growth at an air-liquid interface. A) Schematic of PCL and ML overlying an epithelial cell monolayer (left panel), with z locations indicated for confocal detection of fluorescence recovery in the PCL and ML (right panel). B) ASL depth determined by z-scanning confocal microscopy: representative ASL fluorescence profiles for non-CF and CF cultures (left panel), with summary of averaged ASL depths (right panel; means ± se, n=10 cultures). C) Representative fluorescence recovery curves for 70-kDa FITC-dextran in non-CF and CF cell cultures made at indicated locations (3 curves from different cultures for each condition).