Table 2. Thermodynamic Parameters of RF and RF Antagonists Binding with RfBP in PBS (pH 7.4) at 25 °C.

system	na	KDa (nM)	ΔHa (kJ mol−1)	ΔG (kJ mol−1)	ΔS (kJ mol−1 K−1)
RF vs RfBP	0.78 ± 0.02	5.0 ± 0.7	−91.2 ± 5.7	−47.5	−0.15
lumiflavin vs RfBP	1.08 ± 0.07	61 ± 3.5	−48.2 ± 7.2	−41.2	−0.02
quinacrine vs RfBP	0.90 ± 0.04	264 ± 22.1	−51.6 ± 3.9	−37.5	−0.05
chloroquine vs RfBP	1.06 ± 0.04	2100 ± 180	−40.4 ± 2.9	−32.4	−0.03
perphenazine vs RfBP	no binding observed
chlorpromazine vs RfBP	no binding observed

^aSee Table 1.