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Absence of Jag2 RNA expression in Jag2del2/Jag2del2 embryos. (a,b) Whole mount in situ hybridization for Jag2 transcripts in Jag2del2/+ control littermate (a,c,e,g) and Jag2del2/Jag2del2 (b,d,f,h) embryos at E10.5. Jag2 RNA was not detectable by in situ hybridization in the mutant embryo. (c–h) Higher magnification views. (c,d) Branchial arches. (e,f) Apical ectodermal ridge of forelimb bud. (g,h) Hindlimb bud. Scale bars: a,b, 500 μm; c–g, 500 μm.
