Figure 6. Reactive granulopoiesis was recapitulated in reverse chimeric spleens.
A Total number of splenocytes in reverse chimeric mice 16 weeks post-transplant. B Chimerism of WT CD45.1+ cells in WT and KO recipient spleens. Representative histogram of CD45.1+ cells in the spleens of a reverse chimeric mouse. C Representative FACS plots of mature populations gated on viable, CD45.1+ cells. D Frequencies and absolute numbers of cells in each population. E CFC counts of day 7 colonies from spleens. Statistical analysis by unpaired t-test on n=3 WT:WT and n=4 WT:KO; * p<0.05, ** p<0.01 and *** p<0.005.