Table 1.
Time of LPS injection, hours AEL | Fixation and staining, hours AEL | Percentage stained only in yolk | Percentage stained only in epidermis | Percentage stained in yolk and epidermis | Percentage not stained | Total no. of embryos (n) |
---|---|---|---|---|---|---|
6–9 | 10–13 | 23 (56) | 0 | 0 | 77 (44) | 70 (73) |
9–12 | 13–16 | 23 | 9 | 6 | 62 | 47 |
12–15 | 16–19 | 0 | 88 (97) | 0 | 12 (3) | 40 (57) |
Embryos were injected in the hemocoel or in the perivitelline space (numbers in parentheses).