Table 1.
Sampling points 4 and 5
| Sampling point 4 | HR E1.5 (20 days) | HR E1.16 (28 days) | HR E1.16 with Kn (56 days) |
|---|---|---|---|
| Total Lipid content (mg/g DW) | 33.41 ± 3.66 | 41.55 ± 4.64 | 27.13 ± 3.41 |
| 14:0 | 0.22 ± 0.02 | 0.43 ± 0.05 | 0.43 ± 0.04 |
| 16:0 | 26.53 ± 1.73 | 23.91 ± 1.49 | 22.56 ± 0.67 |
| 18:0 | 2.92 ± 0.30 | 4.10 ± 0.28 | 4.02 ± 0.27 |
| 18:1n-9 | 4.56 ± 0.92 | 5.53 ± 1.00 | 5.61 ± 0.72 |
| 18:1 n-7 | 1.53 ± 0.05 | 1.19 ± 0.09 | 1.03 ± 0.17 |
| 18:2n-6 (LA) | 36.82 ± 0.99 | 41.39 ± 0.95 | 40.16 ± 1.55 |
| 18:3n-6 (GLA) | 13.88 ± 0.78 | 10.48 ± 0.27 | 10.55 ± 0.53 |
| 18:3n-3 (ALA) | 4.24 ± 0.20 | 3.54 ± 0.25 | 4.61 ± 1.75 |
| 18:4n-3 (SDA) | 1.26 ± 0.45 | 0.68 ± 0.00 | 0.84 ± 0.30 |
| 20:0 | 0.29 ± 0.06 | 0.40 ± 0.08 | 0.37 ± 0.05 |
| 22:0 | 2.45 ± 0.31 | 2.58 ± 0.36 | 2.50 ± 0.32 |
| 24:0 | 1.94 ± 0.49 | 2.49 ± 0.28 | 2.72 ± 0.77 |
| Unknown | 2.36 ± 0.35 | 2.64 ± 0.08 | 2.65 ± 0.15 |
| Fatty acids (% of total lipid content) | 22.17 ± 4.23 | 23.96 ± 4.02 | 18.12 ± 2.43 |
| GLA and SDA | 15.14 ± 1.23 | 11.16 ± 0.27 | 11.39 ± 0.83 |
| Saturated fatty acids | 34.36 ± 1.67 | 33.90 ± 1.92 | 32.61 ± 0.99 |
| Monoene fatty acids | 7.07 ± 0.73 | 7.37 ± 0.82 | 7.17 ± 0.87 |
| n-9 | 5.33 ± 0.66 | 6.04 ± 1.11 | 6.14 ± 0.79 |
| n-6 | 50.70 ± 1.66 | 51.87 ± 0.97 | 50.72 ± 1.06 |
| n-3 | 5.50 ± 0.53 | 4.22 ± 0.25 | 5.45 ± 2.05 |
| n-3/n-6 | 0.11 ± 0.01 | 0.08 ± 0.00 | 0.11 ± 0.04 |
| n-6 Δ6-Desaturation Index | 0.27 ± 0.01 | 0.20 ± 0.01 | 0.21 ± 0.01 |
| n-3 Δ6-Desaturation Index | 0.23 ± 0.06 | 0.16 ± 0.01 | 0.15 ± 0.00 |
| DBI | 1.40 ± 0.06 | 1.35 ± 0.03 | 1.36 ± 0.04 |
| Sampling point 5 |
HR E1.5 (25 days) |
HR E1.16 (35 days) |
HR E1.16 with Kn (70 days) |
| Total Lipid content (mg/g DW) | 35.00 ± 5.40 | 29.75 ± 1.63 | 34.34 ± 5.25 |
| 14:0 | 0.23 ± 0.02 | 0.27 ± 0.06 | 0.38 ± 0.08 |
| 16:0 | 25.04 ± 1.14 | 23.63 ± 0.45 | 25.23 ± 0.27 |
| 18:0 | 2.55 ± 0.21 | 4.06 ± 0.21 | 3.93 ± 0.18 |
| 18:1n-9 | 6.77 ± 1.10 | 6.12 ± 0.44 | 4.01 ± 0.24 |
| 18:1 n-7 | 1.55 ± 0.16 | 0.86 ± 0.23 | 0.99 ± 005 |
| 18:2n-6 (LA) | 34.55 ± 1.97 | 42.89 ± 1.31 | 38.05 ± 0.55 |
| 18:3n-6 (GLA) | 11.77 ± 1.63 | 10.05 ± 0.40 | 11.95 ± 0.31 |
| 18:3n-3 (ALA) | 4.22 ± 0.34 | 3.69 ± 0.23 | 5.12 ± 0.26 |
| 18:4n-3 (SDA) | 0.88 ± 0.21 | 0.58 ± 0.03 | 1.09 ± 0.22 |
| 20:0 | 0.27 ± 0.04 | 0.42 ± 0.02 | 0.46 ± 0.02 |
| 22:0 | 2.51 ± 0.35 | 2.59 ± 0.19 | 2.76 ± 0.09 |
| 24:0 | 2.08 ± 0.60 | 2.25 ± 0.45 | 2.69 ± 0.24 |
| Unknown | 6.08 ± 2.68 | 2.03 ± 0.44 | 2.87 ± 0.17 |
| Fatty acids (% of total lipid content) | 24.53 ± 4.16 | 26.86 ± 3.29 | 27.27 ± 9.10 |
| GLA and SDA | 12.65 ± 1.84 | 10.63 ± 0.43 | 13.04 ± 0.53 |
| Saturated fatty acids | 32.68 ± 2.21 | 33.22 ± 1.21 | 35.45 ± 0.07 |
| Monoene fatty acids | 9.54 ± 1,29 | 7.53 ± 0.55 | 5.48 ± 0.15 |
| n-9 | 7.59 ± 1.15 | 6.66 ± 0.50 | 4.38 ± 0.08 |
| n-6 | 46.32 ± 3.60 | 52.94 ± 1.67 | 50.00 ± 0.47 |
| n-3 | 5.09 ± 0.53 | 4.28 ± 0.23 | 6.21 ± 0.41 |
| n-3/n-6 | 0.11 ± 0.01 | 0.08 ± 0.00 | 0.12 ± 0.01 |
| n-6 Δ6-Desaturation Index | 0.25 ± 0.02 | 0.19 ± 0.00 | 0.24 ± 0.01 |
| n-3 Δ6-Desaturation Index | 0.17 ± 0.02 | 0.14 ± 0.01 | 0.17 ± 0.03 |
| DBI | 1.30 ± 0.08 | 1.37 ± 0.04 | 1.37 ± 0.00 |
Total lipid content and general fatty acid profiles (%) of two cell lines of Echium acanthocarpum hairy roots at sampling points 4 and 5 cultured in B5 nutrient liquid medium at 25°C. n-6 and n-3 Δ6-Desaturation Indexes were calculated as 18:3n-6/(18:3n-6+18:2n-6) and 18:4n-3/(18:4n-3+18:3n-3), respectively. Double Bond Index was calculated as [(% 18:1) +2*(% 18:2) +3*(% 18:3) +4*(18:4)]/100. Values are presented as the average of three replicates (data of samplings 4&5 are presented here, all remaining data sets are given in the Additional file 1. (The different sampling point days are also specified in the Method section).