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. 2001 Mar 27;98(7):3889–3894. doi: 10.1073/pnas.051633898

Figure 3.

Figure 3

(A) PCR DGGE 16S rDNA banding profiles of epiphytic bacteria on citrus Valencia trees either directly extracted for DGGE or extracted after 4.5 days growth in different wells of a BIOLOG plate at 25°C. Lanes Val1, Val2, and Val3 are epiphytic bacteria directly extracted from Valencia orange trees 1, 2, and 3. Lanes: A6 and A10, BIOLOG with β-methyl-d-glucoside; B1, B5, and B9, BIOLOG with pyruvic acid methyl ester; D2, D6, and D10, BIOLOG with d-mannitol; E2, E6, and E10, BIOLOG with n-acetyl-d-glucosamine; G2, G6, and G10, BIOLOG with glucose-1-phosphate; H1, H5, and H9, BIOLOG with α-d-lactose. Lanes B1, D2, E2, G2, and H1 were samples from Valencia tree 1. Lanes A6, B5, D6, E6, G6, and H5 were samples from Valencia tree 2. Lanes A10, B9, D10, E10, G10, and H9 were from Valencia tree 3. (B) PCR DGGE 16S rDNA banding profiles of epiphytic bacteria from citrus Valencia leaves directly extracted for DGGE. Lanes Val1, Val2, and Val3 are epiphytic bacteria directly extracted from Valencia orange trees 1, 2, and 3. (C) PCR DGGE 16S rDNA banding profiles of phylloplane bacteria directly extracted from Valencia trees or extracted after 4.5 days growth in different wells of a BIOLOG plate at room temperature. Lanes: A11, BIOLOG with d-galactonic acid γ-lactone; B6 and B10, BIOLOG with d-xylose; B7 and B11, BIOLOG with d-galacturonic acid; B8 and B12, BIOLOG with l-asparagine; C5, BIOLOG with Tween 40; C10, BIOLOG with I-erythritol; D4 and D12, BIOLOG with l-serine; F6, BIOLOG with d-glucosaminic acid; F7, BIOLOG with itaconic acid; F12, BIOLOG with glycyl-l-glutamic acid; G1 and G9, BIOLOG with d-cellobiose; H8, BIOLOG with putrescine. Lanes D4 and G1 were samples from Valencia tree 1. Lanes B6, B7, B8, C5, F6, F7, and H8 were samples from Valencia tree 2. Lanes A11, B10, B11, B12, C10, D12, F12, and G9 were from Valencia tree 3. Lane Val3 is epiphytic bacteria directly extracted from Valencia orange trees 3.