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. 2011 Jun 14;6(6):e20585. doi: 10.1371/journal.pone.0020585

Figure 4. Purified histidine-tagged Vag8 passenger domain binds C1inh.

Figure 4

Shown are means ± SD of a representative ELISA done in triplicate, where Nunc MaxiSorp® flat-bottom 96 well plates were coated with increasing two-fold concentrations of purified histidine-tagged Vag8 passenger or purified histidine-tagged BrkA passenger, and after blocking non-specific binding, incubated with either 10 µg/ml purified C1inh (A), or 2% normal human serum diluted in 1× Veronal buffer (B). For each graph shown, two independent experiments were done.