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. 2011 Jun 14;6(6):e20899. doi: 10.1371/journal.pone.0020899

Figure 3. The concurrent combination of BEZ235 and RAD001 effectively inhibits colony formation ands growth of NSCLC cell.

Figure 3

The indicated cell lines at a density of approximately 200 cells/well were seeded in 24-well plates. On the second day, the cells were treated with 1 nM RAD001 (RAD), 5 nM BEZ235 (BEZ) or their concurrent combinations (RAD+BEZ). The cells were also treated with 1 nM RAD001 for 6 days followed with 5 nM BEZ235 for another 6 days (RAD→BEZ) or with 5 nM BEZ for 6 days followed with 1 nM RAD001 for another 6 days (BEZ→RAD). After 12 days, the plates were stained for the formation of cell colonies with crystal violet dye. The picture of the colonies was then taken using a digital camera (A) and the colony numbers were counted (B). ***, P<0.001 compared with DMSO control; ###, P<0.001 compared with all other treatments.