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. 2011 Jun 14;6(6):e21175. doi: 10.1371/journal.pone.0021175

Figure 2. LyGDI is a novel binding partner of SHIP.

Figure 2

(A) THP-1 cells were stimulated for the indicated time points using human FcγRIIa/IIb (CD32) antibody followed by goat F(ab')2 anti-mouse IgG. SHIP was immunoprecipitated from resting ‘R’ and activated ‘A’ cells and analyzed by Western blotting (IB) with anti-LyGDI (upper panel). Control IPs were performed using normal mouse IgG. The same membrane was reprobed with anti-phosphotyrosine (pY) (middle panel) and anti-SHIP (lower panel). Similar co-immunoprecipitation assays were performed from cells stimulated using (B) F(ab')2 fragments of FcγRI antibody 32.2 and goat F(ab')2 anti-mouse IgG secondary antibody, and (C) F(ab')2 fragments of FcγRIIa antibody IV.3 and goat F(ab')2 anti-mouse IgG secondary antibody.