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. Author manuscript; available in PMC: 2012 Apr 21.
Published in final edited form as: Org Biomol Chem. 2011 Mar 1;9(8):2784–2789. doi: 10.1039/c0ob01269f

Fig. 1.

Fig. 1

Sequential two-step multienzyme synthesis of α2–3-sialylated β1–3-linked galactoside trisaccharides containing different sialic acid forms and diverse underlying glycans. Enzymes: GalK, E. coli K-12 galactokinase; BiGalHexNAcP, B. infantis D-galactosyl-β1–3-N-acetyl-D-hexosamine phosphorylase; Pm aldolase, P. multocida sialic acid aldolase; NmCSS, N. meningitidis CMP-sialic acid synthetase; PmST1, P. multocida multifunctional α2–3-sialyltransferase. Compounds: Gal, D-galactose; GalNAc, N-acetyl-D-galactosamine; GlcNAc, N-acetyl-D-glucosamine; ATP, adenosine 5′-triphosphate; Man, D-mannose; ManNAc, N-acetyl-D-mannosamine; CTP, cytidine 5′-triphosphate; Sia, sialic acid.