Table 4.
IC50s of Zanamivir and NeuAc2en for activities of human and influenza A virus neuraminidasesa
| Viral Strain | Type | IC50 |
|||
|---|---|---|---|---|---|
| Neu5Acα2–3GalβpNP | Neu5Acα2–6GalβpNP | ||||
| Zanamivir (nM) | Neu5Ac2en (uM) | Zanamivir (nM) | Neu5Ac2en (µM) | ||
| sh/HKWF569/07 | H3N1 | 14.3±1.6 | 10.7±1.4 | ND | ND |
| mal/8212/08 | H6N1 | 6.5±0.3 | 3.8±0.4 | ND | ND |
| wi/HKWF541/07 | H6N5 | 17.2±2.8 | 1.8±0.5 | ND | ND |
| sh/JN950/06 | H10N7 | 10.4±1.4 | 5.0±1.3 | ND | ND |
| gos/HKWF446/07 | H10N7 | 23.6±2.7 | 7.9±1.4 | ND | ND |
| mal/6957/08 | H10N7 | 49.9±3.1 | 23.2±1.5 | ND | ND |
| Q/29209-1/93 | H9N2 | 59.9±3.6 | 48.3±5.8 | 12.8±1.0 | 7.2±1.3 |
| ck/SF3/99 | H9N2 | 20.3±1.3 | 6.7±0.8 | ND | ND |
| Qa23CkA10 | H9N2 | 9.5±1.6 | 6.4±1.2 | ND | ND |
| A/PR8* | H1N1 | 3.3±0.2 | 2.6±0.5 | 3.2±0.5 | 1.8±0.4 |
| A/Udorn72* | H3N2 | 94.4±6.2 | 17.8±4.7 | 78.8±6.8 | 11.3±2.7 |
a
Unpurified avian influenza A viruses (12.8 HAU mL−1) and purified human viruses (A/PR8, 1 × 104 HAU mL−1; A/Udon, 5 × 103 HAU mL−1) were used. The reactions were carried out at 37 °C for 60 min with 1 mM of substrate.
*
Purified human influenza A virus; ND, not determined.