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The current reprogramming strategies used to induce pluripotent stem cells from adult somatic cells. The first method introduced was the viral delivery system involving the use of adenovirus, retrovirus and lentivirus. But since then, non-viral methods have been explored. For example, episomal plasmids for gene delivery and cre-loxP and piggyBac transposon are used as the excision strategy. Protein-tagging, cell culture manipulations and miRNAs are non-DNA modification methods
