Fig. 6.

DNA methylation and gene chip analysis in FY-phES-018. a Detection of the methylation status of SNRPN revealed that FY-phES-018 at passage 35 and 60 showed only a methylated pattern (174 bp), and no unmethylated pattern could be detected (100 bp). Both methylated maternal SNRPN and unmethylated paternal SNRPN were detected in FY-hES-7. Relative expression analysis of maternally expressed genes (b) and paternally expressed genes (c) by gene chip analysis in FY-phES-018 cells with normal hESCs used as a calibrator. Black bars and gray bars are the relative gene expression levels between FY-phES-018 and the calibrator. The gray bars with an asterisk (*) in (b) indicate maternally expressed genes down-regulated in FY-phES-018 with a ratio of less than 0.5. In addition, the gray bars with an asterisk (*) in (c) indicate paternally expressed genes up-regulated in FY-phES-018 with a ratio of more than 1.5