Fig. 2.
Results from initial enrichment experiments. Agarose gel electrophoresis analysis of PCR products obtained using DNA templates produced after reverse transcription of mRNA recovered after different numbers of rounds of IgG affinity selection of ternary complexes containing ZWT-ABD proteins. Lane 1: marker DNA; lane 2: PCR product obtained after reverse transcription and amplification of an initial 1:1000 mixture of ZWT-ABD mRNA in a background of ZTaq mRNA; lane 3: PCR product obtained after a first round of enrichment; lane 4: PCR product obtained after a second round of enrichment. Arrows indicate marker DNA bands and the expected sizes for amplicons corresponding to amplification of ZWT-ABD and ZTaq constructs, respectively