Fig. 6.
Binding studies of Zmab25 with a panel of eight antibody samples. Zmab25 was recombinantly produced as a hexahistidyl (His)6 fusion protein, IMAC purified and immobilized onto a CM5 sensor chip surface and various immunoglobulin samples of a common 100 nM concentration injected. Response curves obtained on samples flowed over the Zmab25 surface are labeled as follows: anti-human interferon-γ mIgG1 mAb3 (gray diamonds), anti-human apolipoprotein mIgG1 mAb2 (gray squares), anti-human apolipoprotein mIgG1 HDL110 (gray circles), and anti-special AT-rich sequence-binding protein 2 mIgG1 8F11 (gray triangles). Responses obtained for anti-trinitrophenol mIgG2b ab18421, anti-TNFα mouse–human chimera Infliximab, human myeloma IgG1 GTX76676, and mIgG1(λ) S1-68.1 can be seen as flat traces at the bottom of the graph. In a parallel experiment, also the mAb1 reagent was found to be recognized by the Zmab25 variant in this format (data not shown)