Figure 5.

EBNA1 promotes telomere dysfunction via induction of ROS. Ramos and Ramos-E1 were cultured in the presence of the ROS scavenger Ebselen for 1 week before the assays. (a) Representative micrographs illustrating the effect of Ebselen on the presence of pH2AX foci (upper panel) and APBs detected by PML (green) and telomere (red) colocalization (lower panel, arrows). (b) Quantification of APBs in control and Ebselen treated Ramos-E1. Mean±s.d. of three experiments. ^*P⩽0.01 (c) Ebselen treatment promotes the relocalization of TRF2 at telomere. Quantification of telomere and telomere localized TRF2 signals was performed as described in the legend of Figure 4c. One representative experiment out of three is shown in the figure.