Fig. 3.

Inhibition of p600 does not affect cell proliferation, apoptosis, and colony-formation capacity under anchorage-dependent conditions. a Proliferation assay under anchorage-dependent conditions. There was no significant difference between p600-targeting siRNA-transfected MKN45 cells and control siRNA-transfected MKN45 cells in terms of cell proliferation under anchorage-dependent conditions. b Proportion of apoptotic cells in (i) control siRNA-transfected MKN45 cells and (ii) p600-targeting siRNA-transfected MKN45 cells as analyzed by flow cytometry. There was no significant difference between p600-targeting siRNA-transfected MKN45 cells and control siRNA-transfected MKN45 cells in terms of apoptotic cells proportion. c Macroscopic appearance of colonies formed under anchorage-dependent conditions; (i) control siRNA-transfected MKN45 cells and (ii) p600-targeting siRNA-transfected MKN45 cells. (iii) Data graph. Data are expressed as mean ± SD. There was no significant difference between p600-targeting siRNA-transfected MKN45 cells and control siRNA-transfected MKN45 cells in terms of colony-formation capacity under anchorage-dependent conditions