Figure 2.

Response of Em reporter constructs to ABI3 effector. (A) Em, ΔRI and ΔRII reporter constructs, and (B) mABRE, mRY and mABRE + mRY were introduced into Physcomitrella protonemal tissue with or without the ABI3 effector construct by particle bombardment. After bombardment, the protonemal tissue was incubated with (black bars) or without (white bars) 10 µM ABA for 24 h, and the protein extracts were used for GUS and LUC assays. Bars indicate the relative promoter activity ± SE (n = 4).