Table 1.
Primer | Primer sequence (5′-3′) | Annealing temperature | Total number of amplification cycle | Amplicon size (bp) |
ACD2-forward | AGTCCATGGAAGACCACGAC | 60°C | 23 | 450 |
ACD2-reverse | AGCACAAGCGACTTGGAACT | |||
CAD1-forward | TCAACGCCTAGCTTTGCTCCAG | 60°C | 26 | 480 |
CAD1-reverse | CTTGAGCAAAGCCATGCTCGTTGG | |||
UBQ-forward | TCGTAAGTACAATCAGGATAAGATG | 55°C | 22 | 210 |
UBQ-reverse | CACTGAAACAAGAAAAACAAACCCT |