Skip to main content
. 2010 Sep 1;5(9):1088–1098. doi: 10.4161/psb.5.9.11823

Table 1.

Specific primers, annealing temperatures and total numbers of amplification cycles used for RT-PCR

Primer Primer sequence (5′-3′) Annealing temperature Total number of amplification cycle Amplicon size (bp)
ACD2-forward AGTCCATGGAAGACCACGAC 60°C 23 450
ACD2-reverse AGCACAAGCGACTTGGAACT
CAD1-forward TCAACGCCTAGCTTTGCTCCAG 60°C 26 480
CAD1-reverse CTTGAGCAAAGCCATGCTCGTTGG
UBQ-forward TCGTAAGTACAATCAGGATAAGATG 55°C 22 210
UBQ-reverse CACTGAAACAAGAAAAACAAACCCT